Synergy between the phage endolysin LysH5 and nisin to kill Staphylococcus aureus in pasteurized milk

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Title : Synergy between the phage endolysin LysH 5 and nisin to kill Staphylococcus 1 aureus in pasteurized milk

26 27 Phage-encoded endolysins are recently considered as new biocontrol tools to inhibit 28 pathogens in food. In this work, we have studied the ionic requirements for optimal lytic 29 activity of LysH5, the endolysin encoded by the staphylococcal bacteriophage phi30 SauS-IPLA88. LysH5 activity was inhibited by the presence of Mn and Zn and 31 enhanced by Ca, Mg and NaCl. When LysH5 was combin...

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The aim of this study was to evaluate the antibacterial effect of nisin-loaded chitosan/alginate nanoparticles as a novel antibacterial delivery vehicle. The nisin-loaded nanoparticles were prepared using colloidal dispersion of the chitosan/alginate polymers in the presence of nisin. After the preparation of the nisin-loaded nanoparticles, their physicochemical properties such as size, shape, ...

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Intravitreal injection of the chimeric phage endolysin Ply187 protects mice from Staphylococcus aureus endophthalmitis.

The treatment of endophthalmitis is becoming very challenging due to the emergence of multidrug-resistant bacteria. Hence, the development of novel therapeutic alternatives for ocular use is essential. Here, we evaluated the therapeutic potential of Ply187AN-KSH3b, a chimeric phage endolysin derived from the Ply187 prophage, in a mouse model of Staphylococcus aureus endophthalmitis. Our data sh...

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Are Phage Lytic Proteins the Secret Weapon To Kill Staphylococcus aureus?

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most threatening microorganisms for global human health. The current strategies to reduce the impact of S. aureus include a restrictive control of worldwide antibiotic use, prophylactic measures to hinder contamination, and the search for novel antimicrobials to treat human and animal infections caused by this bacterium. The last ...

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Effective Removal of Staphylococcal Biofilms by the Endolysin LysH5

Staphylococcal biofilms are a major concern in both clinical and food settings because they are an important source of contamination. The efficacy of established cleaning procedures is often hindered due to the ability of some antimicrobial compounds to induce biofilm formation, and to the presence of persister cells, a small bacterial subpopulation that exhibits multidrug tolerance. Phage lyti...

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ژورنال

عنوان ژورنال: International Journal of Food Microbiology

سال: 2010

ISSN: 0168-1605

DOI: 10.1016/j.ijfoodmicro.2010.04.029